Methods for Studying Mononuclear Phagocytes

Methods for Studying Mononuclear Phagocytes

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Methods for Studying Mononuclear Phagocytes is a practical guide to the study of mononuclear phagocytes that brings together various well-established and useful methods for examining these cells. The technical protocols have been made detailed, specific, practical, and inclusive of the necessary mystique for immediate and direct application in the laboratory. The book is divided into 11 parts arranged according to the sequence of steps that would generally be followed to study a given population of mononuclear phagocytes: (I) methods for obtaining and culturing populations of human and animal mononuclear phagocytes; (II) methods for separating populations of leukocytes to enrich or deplete their content of mononuclear phagocytes; (II) criteria and techniques for identifying mononuclear phagocytes; (IV) methods for quantifying the number of mononuclear phagocytes; (V) techniques for studying the morphology of mononuclear phagocytes; (VI) methods for quantifying the biochemical constituents of mononuclear phagocytes; (VII) methods of quantifying phagocytosis, pinocytosis, and chemotaxis; (VIII) methods for quantifying the secretory products of mononuclear phagocytes; (IX) procedures for quantifying the destruction of tumor cells and of microorganisms by mononuclear phagocytes; (X) methods for studying the cell biology of mononuclear phagocytes; and (XI) techniques for studying mononuclear phagocytes in vivo, including procedures for estimating their kinetics, accumulation, identification, and microbicidal properties.using 0.10 M potassium phosphate buffer at pH 6.0 (instead of Tris) and fast garnet GBC at 10X the above concena€” trations (47). Under these ... We use the nitro blue tetrazolium (Nitro BT) method dea€” veloped by the Seligman group (50, 23). B. Reagents Nitro blue ... For stability the reader is referred to Section II.D.2.

Title:Methods for Studying Mononuclear Phagocytes
Author: Dolph Adams
Publisher:Elsevier - 2012-12-02

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