Skeletal Research: An Experimental Approach

Skeletal Research: An Experimental Approach

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Skeletal Research, An Experimental Approach discusses experimental studies on bone cell and tissue biochemistry, biomechanics, isolation, and methods of analysis. Divided into four parts encompassing 21 chapters, this book describes the advantages and limitations of approaches and options available for bone laboratories. The opening part of this book describes the isolation, characterization, and methods of analysis of bone and cartilage cells and their organ cultures. This description is followed by discussions on the intermediary metabolism of growth and articular cartilage; the isolation of plasma membranes from calcified connective tissues; and the aspects of lipidology as it applies to calcified tissue. The third part of this book focuses on the chemical, histochemical, and pathophysiological studies of bone as tissue. This text includes significant chapters on bonea€™s biomechanics and bioelectricity; bone mineral composition; collagen biochemistry; calcium metabolism; and blood flow. Studies on bone and cartilage as circadian systems in rats and mice, emphasizing the importance of this aspect of experimental design, are covered in the concluding part of this book. This text is an ideal resource to experienced researchers and young investigators who wish to expand their technical knowledge on bone research.... 144 Acid mucopolysaccharides, histochemical localization, 159 Acid phosphatase, 8, 144, 146 in isolated bone cells, determination, ... 372-389 schematic diagram of, 370 Ag/AgCl electrode, 348-349 operational procedures, 358 Agarose, cell growth surface, 70 Age, ... 506-507 AGP, see Glycoprotein, anionic Albumin, labeled, in blood flow measurements, 407, 412 [a#39;3a#39;1Albumin, 482 [99aquot;a#39;TclAlbumin, anbsp;...

Title:Skeletal Research: An Experimental Approach
Author: David J. Simmons
Publisher:Elsevier - 2012-12-02

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